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Glycolysis: Substrate-Level Phosphorylation, NAD⁺ Regeneration, and Regulation · Semester 2

Anaerobic Respiration: Metabolic Rationale, Fermentation Pathways, and Lactate Clearance

Students will apply Mendel's laws of inheritance to predict phenotypic and genotypic ratios in offspring, using Punnett squares.

Key Questions

  1. Explain why anaerobic respiration produces substantially less ATP than aerobic respiration per glucose molecule, and justify why organisms resort to fermentation despite its energetic inefficiency when oxygen availability is limiting.
  2. Compare lactate fermentation in animal muscle with alcoholic fermentation in yeast at the biochemical level, explaining how each pathway regenerates NAD⁺ to sustain continued glycolytic flux in the absence of oxidative phosphorylation.
  3. Evaluate the metabolic consequences of lactate accumulation in exercising skeletal muscle and critically assess the oxygen debt concept, referencing the Cori cycle as the mechanism by which the liver removes lactate and restores muscle glycogen.

MOE Syllabus Outcomes

MOE: Genetic Basis of Variation - MS
Level: JC 1
Subject: Biology
Unit: Glycolysis: Substrate-Level Phosphorylation, NAD⁺ Regeneration, and Regulation
Period: Semester 2

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