Activity 01
Inquiry Circle: Enzyme Activity Lab
Using hydrogen peroxide and potato (containing the enzyme catalase), groups test decomposition rate with and without the catalyst. They vary one condition (temperature or pH) to observe how the enzyme's three-dimensional structure affects its function. Each group constructs energy diagrams for the catalyzed and uncatalyzed reactions and explains the activation energy difference in their written analysis.
Explain the role of activation energy in a chemical reaction.
Facilitation TipDuring the Enzyme Activity Lab, circulate to ensure students measure enzyme activity under consistent conditions, such as temperature and substrate concentration, to isolate the effect of the catalyst.
What to look forProvide students with two reaction coordinate diagrams, one for an uncatalyzed reaction and one for a catalyzed reaction. Ask them to label the activation energy for both and write one sentence explaining the difference.