Recombinant DNA Technology · Summer Term

DNA Cloning and Amplification

Techniques for isolating, amplifying, and transferring DNA fragments using PCR and vectors.

Key Questions

  1. 1How has the Polymerase Chain Reaction revolutionized forensic science and medical diagnostics?
  2. 2What are the technical challenges of inserting a eukaryotic gene into a prokaryotic plasmid?
  3. 3Why are marker genes necessary when identifying successfully transformed cells?

National Curriculum Attainment Targets

A-Level: Biology - Recombinant DNA TechnologyA-Level: Biology - DNA Cloning
Year: Year 13
Subject: Biology
Unit: Recombinant DNA Technology
Period: Summer Term

Suggested Methodologies

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